Reversal of proplatelet elongation by nocodazole addition during proplatelet elongation. Frames from experiment shown in supplemental Video 3 were selected from (top to bottom) before (13 minutes) and during elongation (21 minutes), after addition of nocodazole (at 21 minutes and 20 seconds), during effect of nocodazole (25 minutes), and at the start (28 minutes), during the progress (31 minutes), and at the completion (38 minutes) of elongation reversal. Notice the modifications between the cell body and its anchorage point (white arrow) during different steps. Confocal microscope analysis of indirect immunofluorescence labeling with anti-tubulin antibody of an MK extending proplatelet before and after nocodazole treatment. MKs exhibit diffuse staining after action of nocodazole, indicating tubulin depolymerization. Bars represent 10 μm.