GATA3 regulates IL-13 expression in classical Hodgkin lymphoma cell lines. (A) Cell culture supernatant of siSCR, siGATA3 no. 1, and siGATA3 no. 2 transduced L-1236 cells 3, 5, and 7 days after infection were analyzed for IL-13 concentration by enzyme-linked immunosorbent assay in triplicate. Statistical significance was determined using a 2-tailed t test. (B) Protein lysates from cells corresponding to cell culture supernatants in (A) were prepared and 100 μg of protein were loaded onto SDS-PAGE. GATA3 abundance was detected with antibody sc-268. Actin served as loading control. One representative experiment of 3 independent experiments is shown.