Flow cytometric analysis of CD34, CD7, and CD14 expression on macaque cord blood cells following 2 weeks of culture under different conditions. Data from 1 representative experiment is shown. The highest level of CD34 expression is seen when DL coculture and HOXB4 are used together. Cells cultured in direct contact with DL-expressing OP9 cells show the highest level of CD7 expression; addition of HOXB4 does not appear to affect CD7 expression. Furthermore, cells cultured with DL conditioned media show greater inhibition of myeloid differentiation than control cultures, but not as high as cells cultured in direct contact with DL-expressing OP9 cells.