Effect of endogenous APC on the number of B16F10 pulmonary tumor foci in C57Bl/6 mice. (A) Mice were treated intraperitoneally with 200 μg of an antibody that blocks both anticoagulant and signaling properties of APC (MPC1609; ■) or a control antibody (MCO1716; □) at 30 minutes before the administration of 3.5 × 105 B16F10 melanoma cells into the lateral tail vein. Antibody administration was repeated at 48 and 96 hours after melanoma cancer cell inoculation. Mice were killed at 14 days after cancer cell injection, and the number of tumor foci at the surface of the lungs was determined. Error bars represent means ± SEMs (n = 6-8), ***P < .001. (B) Representative lungs of mice treated with MCO1716 and MPC1609, respectively. Dark dots in the lungs represent melanoma tumors.
