The resolution of bivalent domains during differentiation is coupled to their association with distinct epigenetic signatures in HSCs. (A) The percentage of promoters changing from a bivalent chromatin state in HSCs after differentiation into MPPs, PreMegEs, and T cells. Promoters either remained bivalent (black), lost H3K27me3 (green), lost H3K4me3 (orange), remained both H3K4me3/H3K27me3 without overlapping peaks (dark gray), or lost both marks (light gray). (B) Resolution of bivalent histone modification profiles at the Zfp536, Zfp28, and Zfp694 promoters located on chromsome 7 in HSCs after differentiation into MPPs, PreMegEs, and T cells, respectively. (C) Subsequent histone modification and PolII profiles for HSC bivalent promoters that lose H3K4me3, H3K27me3, both, or remain bivalent in MPPs (blue line), PreMegEs (red line), and T cells (gray line). For each plot, the x-axis shows the tiled promoter regions, and the y-axis is the average number of peaks at promoters. (D) Subsequent histone modification and PolII profiles for HSC bivalent promoters also occupied with H3K9me3, H3K79me2, H3ac, and PolII that lose H3K4me3, H3K27me3, both, or remained bivalent in MPPs (blue), PreMegEs (red), or T cells (gray).