TLR7 triggers expression of IFN-inducible genes in a type I IFN–independent manner. GEN2.2 cells were untreated or stimulated with Flu or CL097 for different times. Supernatants were collected for evaluation of (A) IFN-α and (B) CXCL10 production. (C) RNA was extracted for measurement of MxA transcription levels by real-time PCR. (D) TRAIL expression was evaluated by flow cytometry. GEN2.2 cells were untreated or stimulated with Flu or CL097 in the absence or presence of anti–IFN-α/β and anti–IFN-α/βR neutralizing antibodies for 4 hours. (E) TRAIL expression was evaluated by flow cytometry, and (F) CXCL10 production was measured in culture supernatants by CBA. Data shown are the means and SDs from duplicate values from 2 independent experiments except data in panel C, which are representative of 2 independent experiments.