Cytotoxic ability of a human NK-cell line from reconstituted NSG mice. (A) Comparison of NKp46, KIR, perforin, and granzyme B (GrzB) expression of a reconstituted mouse-derived human NK-cell line with a line of human adult PB NK cells. (B) Cytotoxicity of the human NK-cell line from reconstituted NSG mice against K562. Loss of membrane integrity of PKH26-labeled K562 cells as a measure of cytotoxicity was assessed by To-Pro-3 iodide staining of DNA, which is blocked by intact cell membranes. Cytotoxicity was evaluated at the indicated NK:K562 (E:T) ratios. One representative of 6 experiments with 3 different NK-cell lines from 3 mice is shown. Numbers in plots represent frequencies within gates.