NK-cell subset development in NSG mice in comparison to human adult and CB. CD3⁻CD56⁺ human NK cells from spleens of reconstituted NSG mice were analyzed for KIR, CD94, NKG2D, CD16, NKG2A, and NKp46 expression (A). One representative of 12 mice is shown. CD3⁻NKp46⁺ human NK cells from spleens of reconstituted NSG mice were analyzed for CD56, CD16, and NKG2A expression (B). A representative staining from 1 of 12 mice is shown. CD16, KIR, CD127, and CD117 expression on CD3⁻NKp46⁺CD56⁻ cells of hu-NSG mice was analyzed (C). One representative of 3 stainings is displayed. In comparison, CD56 and CD16 expression was analyzed on CD3⁻NKp46⁺ NK cells from human adult (PB) and CB. One representative example (D) and composite data (E) of 8 experiments are shown. CFSE-labeled CD3⁻NKp46⁺CD56⁻ cells of hu-NSG mice were adoptively transferred into hu-NSG mice, which had been reconstituted with autologous CD34⁺ HPCs. CD56 expression on the recovered NKp46⁺ CFSE-labeled cells from recipient spleens was analyzed after 48 hours by flow cytometry (F). Gray shaded histogram of post-sort CD3⁻NKp46⁺CD56⁻ cells in comparison to black histogram of post-sort CD56⁺ (left panel). White histogram of recovered cells in comparison to gray shaded histogram of cells that were transferred (right panel). One representative of 2 experiments is shown. Numbers in plots represent frequencies within gates or quadrants.