IL-27 inhibition of T-cell HIV includes an IFN intermediate. (A) IL-27 induction of IFN-α in T cells at 0.5 to 4 hours (4 hours shown; n = 2), without corresponding increase in APOBEC3A until 24 hours but no increase in APOBEC3G (inset; n = 3). (B) Isolated T cells were blasted with phytohemagglutinin (3 days) and treated with IFN-α, IL-27, or IFN-α plus IL-27 once at 2 hours after infection with HIV_IIIB and HIV infection monitored by p24 ELISA after 7 days (*P = .04, **P < .01, ***P = .002 compared with HIV control; n = 2).