The CD94^highCD56^dim NK-cell subset shows an intermediate level of granzyme B and perforin 1 gene expression and CD94-mediated redirected killing compared with the CD94^highCD56^bright and the CD94^lowCD56^dim NK-cell subsets. (A-B) Real-time PCR analysis of GZMB (A) and PRF1 (B) using cDNA from the freshly sorted CD94^highCD56^bright, CD94^highCD56^dim, and CD94^lowCD56^dim NK-cell subsets. Data are summarized from 6 donors, and the error bars represent SD. (C) ⁵¹Cr cytotoxicity analysis of the aforementioned sorted 3 NK-cell subsets against the murine cell line P815 in the presence of the purified anti–human CD94 mouse mAb. Data are summarized from 3 donors, and the error bars represent SD.