TSP-1 overexpressed, induced by angiostatin, inhibits melanoma tumor cell growth. (A) HUVECs overexpressed TSP-1 by angiostatin treatment, whereas tumor cells do not. (B) HUVECs secreted TSP-1 into cell culture medium (Sup). (C) Fluorescence-activated cell sorter (FACS) analysis showed that A2058 melanoma cells express CD36 on the surface. (D) Melanoma tumor cell proliferation was inhibited by 10 μg/mL recombinant TSP-1 treatment. (E) TUNEL assay showed more apoptosis of tumor cells after rTSP-1 treatment. (F) c-Myc protein level of tumor cells was decreased after treatment with TSP-1–rich HUVEC culture conditional medium (CM) or serum-free medium (SF).