gata2^hi cells display the morphologic, histochemical, and ultrastructural properties of eosinophils. gata2^hi and mpx^hi cells were FACS sorted (purity ≥ 95%) from WKM (A-B) or IPEX (B). (A) Purified gata2^hi (EOS) and mpx^hi (NEU) cells were stained with hematoxylin and eosin, WG, PAS (red precipitate), MPX (brown precipitate), and TB (purple precipitate). Bar represents 5 μm. (B) Purified gata2^hi cells were subjected to double staining with MPX and PAS or TEM analysis. A representation of each nuclear morphology (myelocyte, metamyelocyte, band, and polymorphonuclear) is shown as observed by cytospin (left) or TEM (right) analysis. Based on cytochemical analyses, the relative abundance (percentage) in either IPEX (top value) or WKM (bottom value) is provided for each morphologic class. Bar represents 2 μm. N indicates nucleus. Data are mean ± SD (n = 10).