Recruitment of Brg-1 and other factors to the c-myc promoter by NFATc1. (A) DLBCL DS cells (NFATc1−) were infected with a retroviral vector containing a mutant NFATc1 (caNFATc1-GFP) or vector containing only GFP (negative control). After 48 hours of incubation, cells were fixed and analyzed for protein binding to the NFAT DNA-binding site in the c-myc promoter by ChIP–Q-PCR with antibodies to NFATc1, p65, c-rel, Brg-1, Brm, STAT3, and IgG (negative control). The data were analyzed by the ChIP–Q-PCR Data Analysis Template. Data represent 3 independent experiments. (B) DLBCL MS and SUDHL-4 cells (NFATc1+) were transfected with the NFATc1 shRNA plasmid or control vector. Forty-eight hours after transfection, cells were fixed and analyzed for protein binding to the NFAT DNA-binding site in the c-myc promoter by ChIP–Q-PCR with antibodies to NFATc1, p65, c-rel, Brg-1, Brm, STAT3, and IgG (negative control). The data were analyzed by the SuperArray ChIP–Q-PCR Data Analysis Template. Data represent 3 independent experiments.