Figure 3
Figure 3. Donor cell origin of an oral squamous cell carcinoma occurring after bone marrow transplantation shown by STR-PCR of laser-microdissected cells in 2 sex-matched transplant recipients. (A top) Case III microscopic pictures of sequential cell-by-cell laser-microdissection of p53-positive tumor cells (↙). p53-positive tumor cells are surrounded by a yellow line before laser microdissection (left); the laser microdissection has been performed for the 2 upper tumor cells (middle); the laser microdissection has been performed for all surrounded p53-positive tumor cells (right). Following are microsatellite analyses that used D8S261, D8S1820, and p53CA STR sequences. For the locus D8S261, the microdissected tumor cells and inflammatory cells of donor origin were similarly heterozygous (134 and 136 base peaks), whereas microdissected epidermal cells of recipient origin were differently heterozygous (128 and 140 base peaks). For the locus D8S1820, the microdissected tumor cells and inflammatory cells of donor origin were similarly heterozygous (104 and 106 base peaks), whereas microdissected epidermal cells of recipient origin were differently heterozygous (106 and 113 base peaks). For the locus p53CA, the microdissected tumor cells and inflammatory cells of donor origin were similarly heterozygous (122 and 124 base peaks), whereas microdissected epidermal cells of recipient origin were differently heterozygous (103 and 118 base peaks). (B) Case IV microscopic pictures of p53-positive tumor sheets invading the lamina propria (left), and at high magnification with successive steps of laser-microdissection (middle and right). Following are microsatellite analyses that used D7S490, D17S855, and D9S162 STR sequences. For the locus D7S490, the microdissected tumor cells and inflammatory cells of donor origin were similarly heterozygous (93 and 103 base peaks), whereas microdissected epidermal cells of recipient origin were differently heterozygous (106 and 117 base peaks). For the locus D17S855, the microdissected tumor cells and inflammatory cells of donor origin were similarly heterozygous (154 and 156 base peaks), whereas microdissected epidermal cells of recipient origin were differently heterozygous (145 and 154 base peaks). For the locus D9S162, the microdissected tumor cells and inflammatory cells of donor origin were similarly homozygous (181 base peak), whereas microdissected epidermal cells of recipient origin were heterozygous (181 and 191 base peaks). Images in panels A and B were viewed with a PALM laser catapulted microdissector system (PALM, Bernried, Germany) on an Olympus IX81 microscope using an Olympus LUCPlanFl 40×/0.6 NA objective and taken with a digital camera using PALM Robo software version 3.

Donor cell origin of an oral squamous cell carcinoma occurring after bone marrow transplantation shown by STR-PCR of laser-microdissected cells in 2 sex-matched transplant recipients. (A top) Case III microscopic pictures of sequential cell-by-cell laser-microdissection of p53-positive tumor cells (↙). p53-positive tumor cells are surrounded by a yellow line before laser microdissection (left); the laser microdissection has been performed for the 2 upper tumor cells (middle); the laser microdissection has been performed for all surrounded p53-positive tumor cells (right). Following are microsatellite analyses that used D8S261, D8S1820, and p53CA STR sequences. For the locus D8S261, the microdissected tumor cells and inflammatory cells of donor origin were similarly heterozygous (134 and 136 base peaks), whereas microdissected epidermal cells of recipient origin were differently heterozygous (128 and 140 base peaks). For the locus D8S1820, the microdissected tumor cells and inflammatory cells of donor origin were similarly heterozygous (104 and 106 base peaks), whereas microdissected epidermal cells of recipient origin were differently heterozygous (106 and 113 base peaks). For the locus p53CA, the microdissected tumor cells and inflammatory cells of donor origin were similarly heterozygous (122 and 124 base peaks), whereas microdissected epidermal cells of recipient origin were differently heterozygous (103 and 118 base peaks). (B) Case IV microscopic pictures of p53-positive tumor sheets invading the lamina propria (left), and at high magnification with successive steps of laser-microdissection (middle and right). Following are microsatellite analyses that used D7S490, D17S855, and D9S162 STR sequences. For the locus D7S490, the microdissected tumor cells and inflammatory cells of donor origin were similarly heterozygous (93 and 103 base peaks), whereas microdissected epidermal cells of recipient origin were differently heterozygous (106 and 117 base peaks). For the locus D17S855, the microdissected tumor cells and inflammatory cells of donor origin were similarly heterozygous (154 and 156 base peaks), whereas microdissected epidermal cells of recipient origin were differently heterozygous (145 and 154 base peaks). For the locus D9S162, the microdissected tumor cells and inflammatory cells of donor origin were similarly homozygous (181 base peak), whereas microdissected epidermal cells of recipient origin were heterozygous (181 and 191 base peaks). Images in panels A and B were viewed with a PALM laser catapulted microdissector system (PALM, Bernried, Germany) on an Olympus IX81 microscope using an Olympus LUCPlanFl 40×/0.6 NA objective and taken with a digital camera using PALM Robo software version 3.

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