Figure 2
Figure 2. Donor cell origin of an oral squamous cell carcinoma occurring after bone marrow transplantation. Tumor of XY genotype occurring in a female patient who received a BM transplant from a male donor (no previous male pregnancy). (A) Combined FISH XY and immunostainings on the same tissue section with antibodies directed against p53 (brown) and cytokeratin (AE1/AE3; blue), showing strong stainings of the tumor cells. Tumor indicates tumor area, delimited through a large broken line; Ca, capillary lumen in the dermis; inflammatory cells, surrounded by short broken lines, in the dermis. The p53/cytokeratin-positive tumor cells are of XY genotype, as shown with green and red arrows on the enlarged overlaid area. (B) High magnification of p53-positive tumor cells (), surrounded by a yellow line before microdissection in the top picture. The holes left after single cell laser microdissection of the same tumor cells are surrounded by the same yellow line and shown by arrowheads in the lower picture. (C,D) Comparison of the profiles of laser-microdissected tumor cells, inflammatory cells (from the donor), and normal epidermal cells (from the recipient) from blocks of the same surgical pieces shows the profile of tumor cells of donor origin with the use of D17S1879 (C) and D1S2892 (D) STR sequences. For the D17S1879 locus (C), the microdissected tumor cells are homozygous, because of the inflammatory cells of donor origin (158 base peak), whereas the microdissected epidermal cells of recipient origin are heterozygous (154 and 158 base peaks). For the D1S2892 locus (D), the microdissected tumor cells and inflammatory cells of donor origin are similarly heterozygous (102 and 117 base peaks), whereas microdissected normal epidermal cells of recipient origin are differently heterozygous (102 and 125 base peaks). Image in panel A was viewed with an Olympus AX70 microscope using Olympus UPLan Fl 4×/0.13 NA and 40×/0.17 NA objectives, and taken with a ColorView III digital camera using Olympus-SIS Cell F software. Image in panel B was viewed with a PALM laser catapulted microdissector system (PALM, Bernried, Germany) on an Olympus IX81 microscope using an Olympus LUCPlanFl 40×/0.6 NA objective and taken with a digital camera using PALM Robo software version 3.

Donor cell origin of an oral squamous cell carcinoma occurring after bone marrow transplantation. Tumor of XY genotype occurring in a female patient who received a BM transplant from a male donor (no previous male pregnancy). (A) Combined FISH XY and immunostainings on the same tissue section with antibodies directed against p53 (brown) and cytokeratin (AE1/AE3; blue), showing strong stainings of the tumor cells. Tumor indicates tumor area, delimited through a large broken line; Ca, capillary lumen in the dermis; inflammatory cells, surrounded by short broken lines, in the dermis. The p53/cytokeratin-positive tumor cells are of XY genotype, as shown with green and red arrows on the enlarged overlaid area. (B) High magnification of p53-positive tumor cells (), surrounded by a yellow line before microdissection in the top picture. The holes left after single cell laser microdissection of the same tumor cells are surrounded by the same yellow line and shown by arrowheads in the lower picture. (C,D) Comparison of the profiles of laser-microdissected tumor cells, inflammatory cells (from the donor), and normal epidermal cells (from the recipient) from blocks of the same surgical pieces shows the profile of tumor cells of donor origin with the use of D17S1879 (C) and D1S2892 (D) STR sequences. For the D17S1879 locus (C), the microdissected tumor cells are homozygous, because of the inflammatory cells of donor origin (158 base peak), whereas the microdissected epidermal cells of recipient origin are heterozygous (154 and 158 base peaks). For the D1S2892 locus (D), the microdissected tumor cells and inflammatory cells of donor origin are similarly heterozygous (102 and 117 base peaks), whereas microdissected normal epidermal cells of recipient origin are differently heterozygous (102 and 125 base peaks). Image in panel A was viewed with an Olympus AX70 microscope using Olympus UPLan Fl 4×/0.13 NA and 40×/0.17 NA objectives, and taken with a ColorView III digital camera using Olympus-SIS Cell F software. Image in panel B was viewed with a PALM laser catapulted microdissector system (PALM, Bernried, Germany) on an Olympus IX81 microscope using an Olympus LUCPlanFl 40×/0.6 NA objective and taken with a digital camera using PALM Robo software version 3.

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