In vitro T-cell immune response to autologous tumor. Results of Elispot assay in 2 representative patients. Blood samples were analyzed at various study time points for T-cell response to autologous leukemia cells and assessed for interferon-γ and granzyme-B production by Elispot. Patient 028-404 showed induction of T-cell response by both interferon-γ (A) and granzyme-B (B) after pre- and posttransplantation immunotherapy. A typical pattern is seen with waning of the pretransplantation response soon after transplantation, followed by recovery with posttransplantation treatments. Patient 028-405 showed induction of T-cell response by both interferon-γ (C) and granzyme-B (D) after posttransplantation immunotherapy only. This response coincided with induction of a tumor DTH skin test response at posttransplantation treatment 8. Analysis time points included: pretransplantation immunotherapy baseline (day 0) and day 14 after the first immunotherapy dose (day 14), before posttransplantation immunotherapy 1 (∼6 weeks after transplantation) and at posttransplantation immunotherapy 4 and 8, and follow-up at month 9 and 12.