Validation of copy-number change in case no. 65. (A) SNP-chip data of chromosome 8 in case no. 65. Top dots are SNP sites as probes and indicate total copy number (CN). Middle line is an average of the copy number and shows gene dosage. Bars are heterozygous (hetero) SNP calls. Bottom 2 lines show allele-specific copy number (AsCN). (B) Duplication of the MYC gene region in case no. 65. Copy number of the MYC gene in case no. 65 was compared with normal genomic DNA with quantitative genomic real-time PCR. Level of the copy number was determined as a ratio between the MYC gene and the reference genomic region 2p21. Results represent mean of 3 experiments plus or minus SD.