Figure 5
Figure 5. The pHi-buffering capacities of Mi.III+ erythrocytes were superior. Fresh RBCs were loaded with fluorescent pH indicator SNARF-1, and its intracellular pH measurement at pHout 7.5 was measured by flow cytometry. In the absence of extracellular bicarbonate, the control cells became more acidified than Mi.III. Depletion of extracellular Cl− maximized HCO3− loading for both Mi.III+ and the control cells, and diminished their pHi differences. The number of donors tested was indicated next to each bar. Data are expressed as mean ± SE; *P < .05.

The pHi-buffering capacities of Mi.III+ erythrocytes were superior. Fresh RBCs were loaded with fluorescent pH indicator SNARF-1, and its intracellular pH measurement at pHout 7.5 was measured by flow cytometry. In the absence of extracellular bicarbonate, the control cells became more acidified than Mi.III. Depletion of extracellular Cl− maximized HCO3− loading for both Mi.III+ and the control cells, and diminished their pHi differences. The number of donors tested was indicated next to each bar. Data are expressed as mean ± SE; *P < .05.

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