The expression levels of GPB and Gp.Mur in Mi.III⁺ RBCs were complementary. (A) Mi.III-specific Gp.Mur probably evolved from homologous gene recombination between GYPA and GYPB, and shows a unique glycophorin B-A-B structure and the antigenic Mur (marked as checkered). The protein sequences of full-length Gp.Mur and GPB, and GPA lacking a cytoplasmic domain, were aligned by the CLUSTALW program. (B) Glycophorin immunoblot of ghost lysates from 6 Mi.III⁺ and 6 non-Mi.III (control) samples (30 mg/lane). Solubilized ghosts were resolved on 10% SDS–polyacrylamide gel electrophoresis. (Left) GPA immunoblot by E4 antibody. (Right) Immunoblot against GAB by E3 antibody. Homozygous Mi.III samples are marked +/+.