Gene expression analysis in menin-deficient hematopoietic progenitors. (A) The following cell populations were purified from the BM of control Cre-ER^Tm−Men1^f/f (WT) or Cre-ER^Tm+Men1^f/f (KO) 4 weeks after administration of tamoxifen: Lin⁻Sca-1^hic-Kit^hi (LSK progenitors, containing HSCs); Lin⁻Sca-1–c-Kit^hiCD16/32^loCD34⁺ (CMP); Lin⁻Sca-1–c-Kit^hiCD16/32^hiCD34⁺ (GMP) and Gr1⁺CD11b⁺ myeloid cells. Quantitative RT-PCR was used to assess the abundance of Men1 and HoxA9 transcripts, relative to Hprt1. (B) Relative expression of HoxA9 and Men1 in total BM from TAM-treated Cre-ER^Tm−Men1^f/f (WT) or Cre-ER^Tm+Men1^f/f (KO) mice. Data are normalized to the WT LSK shown in panel A. (C) Relative expression of Meis1, p18Ink4c, and HoxA10 in control (WT) and menin-deficient (KO) LSK progenitors. Each sample was assayed in triplicate and expressed after normalization to 1 in WT LSK. Data are representative of 2 to 3 individual mice. Error bars represent the 95% confidence interval. * indicates statistical significance (P < .05).