Normal homing of menin-deficient hematopoietic progenitors. (A) Unirradiated hosts: BM from TAM-treated CD45.2⁺Cre-ER^Tm+Men1^f/f or control Cre-ER^Tm−Men1^f/f mice was injected into the tail vein of CD45.1⁺ B6-SJL recipients (2.5 × 10⁷ cells/mouse). Three days later flow cytometry was used to analyze the homing efficiency of CD45.2⁺ donor LSK cells in the host bone marrow. Contour plots are representative of 3 individual recipients in each group. (B) Irradiated hosts: lethally irradiated (900 rads) B6 mice were transplanted with 3 × 10⁷ CFSE-labeled cells from TAM-treated CD45.2⁺Cre-ER^Tm+Men1^f/f or Cre-ER^Tm−Men1^f/f mice. The host BM and spleen were analyzed by flow cytometry 6 hours after transfer. Graphs show the absolute number of CFSE⁺Lin⁻ cells recovered from 2 hind legs (mean + SEM). Contour plots are representative of 4 individual recipients in each group. WT indicates recipients of control cells; and KO, recipients of menin-deficient cells.