Involvement of rab11 in sorting the internalized FVIIa into the recycling compartment. CHO-EPCR cells were transiently transfected with wild-type rab11, constitutively active rab11 (Q70L), or dominant negative rab11 (S25N), and the transfected cells were exposed to AF488-FVIIa for 1 hour at 37°C. The cells were fixed, permeabilized, and immunostained for rab11. The fluorescence of internalized AF488-FVIIa and immunofluorescence of rab11 were analyzed by confocal microscopy.