Intracellular distribution of EPCR in endothelial cells. Permeabilized HUVECs were immunostained with EPCR mAbs and an organelle-specific antibody. (Left panel) EPCR-specific staining. (Middle panel) Organelle-specific staining. (Right panel) The overlay image (colocalization) of organelle-specific marker and EPCR. Organelle-specific antibodies used were as follows: anti-EEA1 and anti-rab5 for early endosomes, anti-LAMP1 for lysosomes, Giantin for the Golgi and rab11 for the recycling compartment. A small portion of the merged image, bordered with the white box, was digitally enlarged to illustrate the colocalization.