Activated Th17 clones can recruit neutrophils through CXCL8 release. (A) CXCL8 expression in phorbol myristate acetate/ionomycin-activated Th17 and Th1 clones, as evaluated by representative intracellular staining (left panel) and shown as the mean (± SE) values of percentage of positive cells (Th17: n = 9; Th1: n = 5, right panel). (B) CXCL8 concentrations in supernatants harvested from resting or αCD3/αCD28-stimulated Th17 and Th1 clones (n = 4). (C) Chemotaxis of neutrophils toward supernatants harvested from αCD3/αCD28-stimulated Th17 clones, in the presence or absence of αCXCL8, or related isotype, Abs (n = 4). *P < .05.