Immunohistochemical analysis of tumor lesions of patient CIP-26 collected during the treatment. (A-G) Melanoma lesions collected during the treatment were stained with HC10 mAb (anti–HLA-B and -C), anti–β2-microglobulin mAb, W6/32 (anti–HLA-I) mAb and anti–HLA-A30 mAb. (A,B) The regressing tumor nodule collected in 2005 after 18 vaccinations, was almost completely negative for HC10 (A), but contained tumor areas stained with anti–β2-microglobulin mAb (B), W6/32 mAb (C) and with an anti–HLA-A30 mAb (D). A progressing tumor lesion collected in 2006, after 22 vaccinations, was not stained by mAb HC10 (E) and by anti–β2-microglobulin mAb (F). Some tumor areas from the progressing lesion were not stained by mAb W6/32 (G) and by an anti–HLA-A30 mAb (H). Objectives, ×200.