Force-dependent kinetics of destabilization and rupture. Time-to-rupture t₁₁ (A,E) or t₂₂ (C,G) or time-to-destabilization t₂₁ (B,F) is plotted versus force measured in the absence (□) or presence of 5 μg/mL ADAMTS-13 without (◊) or with (○) 5mM EDTA for A1A2A3 adsorbed directly on polystyrene dish and pulled by GPIbα (A-C) or captured by preadsorbed anti-His mAb and pulled by CR1 (E-G). Data are mean ± SEM of several tens of measurements for each point. The rate of cleavage as a function of force was calculated by k_cat = 1/t₂₂ − 1/t₂₂⁰ denotes using the respective data in panel C (D) or panel G (H).