Impact of mode of destabilization. (A-B) Comparison of destabilization times (A) or rupture times (B) for molecules with structural destabilization that yielded short (l_d < 35 nm, open bars) and long (l_d > 35 nm, closed bars) length increases in the absence or presence of 5 μg/mL ADAMTS-13 without or with 5mM EDTA. (C-D) Comparison of time-to-destabilization t₂₁ (C) or time-to-rupture t₂₂ (D) measured in the absence (open bars) or presence of ADAMTS-13 without (closed bars) or with (hatched bars) EDTA for molecules with structural destabilization that yielded short or long length increases. Data were acquired by GPIbα-pulling and presented as mean ± SEM of several tens of measurements. P values of Student t test are shown to indicate the statistical significance (or lack thereof) of the differences.