Unilineage erythroid (E) and granulocytic (G) culture of HPCs: NFI-A expression pattern during E and G differentiation/maturation. (A) Unilineage E culture of CB CD34⁺ HPCs. (i) Growth curve, (ii) CD34 and GPA surface marker expression, and (iii) percentage of blasts, proerythroblasts (Blasts), basophilic (Baso), polychromatophilic (Poly), and orthochromatic (Ortho) erythroblasts (mean ± SEM values; n = 7). (B) Unilineage G culture of CB CD34⁺ HPCs. (i) Growth curve, (ii) CD34 and CD15 surface marker expression, and (iii) percentage of blasts, promyelocytes (Promyelo), myelocytes, mature metamyelocytes, and band cells (Meta/band). Mean ± SEM values (n = 8). (C) NFI-A increases in E culture. (Top) Real-time PCR evaluation of NFI-A mRNA. Normalized mean ± SEM values (n = 3). (Bottom) Immunoblot of NFI-A protein. β-Actin was used as loading control. (D) NFI-A decrease in G culture. (Top) Real-time PCR evaluation of NFI-A mRNA. Normalized mean ± SEM values (n = 3). (Bottom) Immunoblot of NFI-A protein.