Binding of HDAC5 to the promoter of FGF2 and Slit2. (A) Overview of primer pairs amplifying the indicated promoter regions relative to the transcription start within the FGF2 and Slit2 genes. (B) Western blot analysis was performed to confirm the immunoprecipitation of myc-tagged overexpressed HDAC5 wt and HDAC5 S259/498A mutant. (C) The ChIP was performed in HUVECs overexpressing myc-tagged HDAC5 wt or HDAC5 S259/498A mutant using an antibody against c-myc. Representative PCR with primers detecting the indicated promoter regions of the FGF2 and Slit2 genes are shown (n = 3). Ab indicates antibody control. (D) Expression of FGF2 mRNA in HUVECs transfected with HDAC5 wt or HDAC5 S259/498A mutant after 48 hours (n = 4). (E) Expression of Slit2 mRNA in HUVECs transfected with HDAC5 wt or HDAC5 S259/498A mutant after 48 hours (n = 3).