Identification of target genes regulated by HDAC5 in endothelial cells. (A) Bioinformatic analysis of the RNA profile of HUVECs transfected with HDAC5 siRNA after 24 hours (n = 3 each). Up- and down-regulated genes in response to HDAC5 siRNA versus scrambled siRNA as a proportion of all analyzed genes are shown in a gene tree analysis. The color scale is shown on the right. The brightness indicates the trust. Blue represents low expression; red, high expression. (B) Summary of selected genes relevant to angiogenesis, which were up-regulated in response to HDAC5 siRNA transfection in the oligonucleotide array. *In case of 2 different oligonucleotides representing one gene, the data were pooled. Because we focused in our following study on Slit2 and FGF2, both targets are highlighted in gray. (C) Quantitative analysis of HDAC5 and selected target gene expression in response to HDAC5 siRNA versus scrambled siRNA after 24 hours and 48 hours using real-time PCR (n = 3; *P < .05 vs scrambled).