Validation experiments using quantitative RT-PCR. Relative expression data presented as box plots for the following genes: VHL, ABI3, ADORA3, and BCL10. Twenty-seven IGHV unmutated and 23 IGHV mutated CLL cases were investigated that had been not included in the methylation array analysis. Values of P are indicated above each box plot. Boxes indicate the interquartile range (25%-75%) with the smaller inner square indicating the median. The whiskers show the minimum and maximum values, except for outliers (○).