A 6-hour E2 exposure to myeloid progenitors yields a significant increase in DCs after 7 days. (A) MPs were stimulated with GM-CSF and 0.1nM E2 in standard medium at time 0. At the indicated times (0-96 hours), ER signaling was blocked by ICI 182,780 (100nM), such that E2 responsiveness was limited to the hour shown on the x axis. All cells were analyzed by flow cytometry on day 7. Shown are the average and range of percentages (left panel) and numbers (right panel) of CD11c⁺ MHCII⁺ DCs in duplicate cultures. (B) Shown are the relative percentages of 2 DC subsets (CD11b^hi Ly6C⁺ and CD11b^int Ly6C⁻) present in cultures harvested on day 7 after E2 exposure for 0, 6, 48, or 168 hours. (C) Shown is the staining of anti-Langerin mAb L31 in DCs in the absence or presence of E2. Langerin⁺ DCs are within the CD11b^int Ly6C⁻ population. Data are representative of 2 independent experiments.