Aldh1a1 is not required for normal hematopoiesis. (A) Cellularity (left) and HSC frequency (right) were determined in young (2-3 months) and old (21-27 months) adult Aldh1a1^+/− and Aldh1a1^−/− mice (n = 6-14 mice per treatment in at least 6 independent experiments). (B) The frequency of colony-forming progenitors in bone marrow (top row) and spleen (second row) of young (left column) and old adult (right column) Aldh1a1^+/− and Aldh1a1^−/− mice (n = 6-7 mice per treatment in 5 independent experiments). (C) The frequency of various subsets of B (B220⁺), T (CD3⁺), erythroid (Ter119⁺), and myeloid (Mac-1⁺Gr-1⁺) lineage cells in the bone marrow (top row) and spleen (bottom row) from young (left column) and old (right column) Aldh1a1^+/− and Aldh1a1^−/− mice (n = 5 mice per treatment in 5 independent experiments). No significant differences were observed between Aldh1a1^−/− and control mice. Error bars represent SD.