ENO-1 expression on blood monocytes and in lung tissue from pneumonia patients. (A) ENO-1 mRNA expression in blood monocytes obtained from pneumonia patients and healthy controls was assessed by real-time PCR. Results are expressed as fold-increase of the ratio of ENO-1/β-actin over control values (healthy controls) and are mean ± SD; n = 5. (B) Western blot illustrating cell-membrane localization of ENO-1 and PLG on blood monocytes from pneumonia patients and healthy controls. Streptavidin beads were used to separate biotinylated membrane proteins from nonbiotinylated cytosolic proteins. The purity of the cell-membrane fraction was assessed by probing the samples for P20S. CD18 was used as loading control. (C) Densitometric analysis of panel B. □ represents healthy subjects, while ■ represents pneumonia patients. **P < .01. (D) Cellular localization of ENO-1 and PLG in monocytes isolated from the blood of pneumonia patients and healthy controls. Original magnification 63×/1.32-0.6 oil objective. Bar size 5 μm. (E) Representative lung tissue sections from pneumonia patients (i-iv) and controls (v-viii) were stained for ENO-1. In lungs from pneumonia patients, strong immunoreactivity for ENO-1 was observed in basal bronchial epithelial cells (i,ii) and mononuclear cells (iii,iv). In lungs from control patients, ENO-1 staining was observed in bronchial epithelial cells (v-viii). Original magnification ×20 (i,iii,v,vii), or ×40 (ii,iv,vi,viii). Bar size 20 μm. Lung sections from 1 representative pneumonia patient and 1 control patient of 5 per group are illustrated. (F) Representative lung tissue sections from pneumonia patients and control patients stained for ENO-1 and PLG. Original magnification ×40. Bar size 20 μm.