ENO-1 mediates the directed migration, transmigration, and invasion of LPS-activated U937 cells and human blood monocytes. U937 cells (left panel) or human blood monocytes (right panel) exposed to 5 μg/mL LPS for 6 hours as well as unstimulated cells were preincubated with 15 μM Lys-PLG in the absence (−) or presence of anti–ENO-1 antibodies (10 μg/mL), IgG control (10 μg/mL), or tranexamic acid (TXA; 10 mM). Subsequently, 3 nM t-PA was added, and the cells seeded onto inserts were analyzed for directed migration through a porous (5-μm) polycarbonate membrane (A), directed transmigration through a monolayer of A549 cells (B), or invasion through Matrigel (C). The inserts were placed into wells without (□) or with MCP-1 (■) and incubated for 12 or 18 hours at 37°C. The cells present in the lower chamber were counted. Data represent mean values ± SD from 4 independent experiments, each performed in quintuplicate; *P < .05; **P < .01; ***P < .001.