Figure 1
Figure 1. Gfi1 coordinately regulates the expression of the HoxA9-Pbx1-Meis1 complex during myeloid progenitor differentiation. (A) Quantitative real-time gene expression analysis of Gfi1 and Gfi1b in sorted CMPs and GMPs from wild-type Lin− bone marrow cells. (B) Quantitative real-time gene expression analysis of HoxA9, Meis1, Pbx1, and Pbx2 in RNA from panel A. (C) Quantitative real-time gene expression analysis of HoxA9, Meis1, Pbx1, and Pbx2 in RNA from wild-type or Gfi1−/− littermate Lin− bone marrow cells. (D) Immunoblot analysis of HoxA9, Pbx1, and Meis1 in protein extracts from wild-type or Gfi1−/− littermate Lin− bone marrow cells. (E) Quantitative real-time gene expression analysis of HoxA9, Meis1, and Pbx1 in RNA from wild-type Lin− bone marrow cells transduced with an empty retroviral vector (Vector) or 1 expressing Gfi1 (Gfi1). (F) Quantitative real-time gene expression analysis of HoxA9, Meis1, and Pbx1 in RNA from Gfi1−/− Lin− bone marrow cells transduced with an empty retroviral vector (Vector) or 1 expressing Gfi1 (Gfi1). (G) Quantitative real-time gene expression analysis of HoxA9, Meis1, Pbx1, and Pbx2 in wild-type Lin− bone marrow cells transduced with retrovirus expressing Gfi1 dominant-negative mutants (P2A or N382S) or an empty vector control (Vector). Error bars indicate SD. *P ≤ .05, **P ≤ .01, ***P ≤ .001.

Gfi1 coordinately regulates the expression of the HoxA9-Pbx1-Meis1 complex during myeloid progenitor differentiation. (A) Quantitative real-time gene expression analysis of Gfi1 and Gfi1b in sorted CMPs and GMPs from wild-type Lin− bone marrow cells. (B) Quantitative real-time gene expression analysis of HoxA9, Meis1, Pbx1, and Pbx2 in RNA from panel A. (C) Quantitative real-time gene expression analysis of HoxA9, Meis1, Pbx1, and Pbx2 in RNA from wild-type or Gfi1−/− littermate Lin− bone marrow cells. (D) Immunoblot analysis of HoxA9, Pbx1, and Meis1 in protein extracts from wild-type or Gfi1−/− littermate Lin− bone marrow cells. (E) Quantitative real-time gene expression analysis of HoxA9, Meis1, and Pbx1 in RNA from wild-type Lin− bone marrow cells transduced with an empty retroviral vector (Vector) or 1 expressing Gfi1 (Gfi1). (F) Quantitative real-time gene expression analysis of HoxA9, Meis1, and Pbx1 in RNA from Gfi1−/− Lin− bone marrow cells transduced with an empty retroviral vector (Vector) or 1 expressing Gfi1 (Gfi1). (G) Quantitative real-time gene expression analysis of HoxA9, Meis1, Pbx1, and Pbx2 in wild-type Lin− bone marrow cells transduced with retrovirus expressing Gfi1 dominant-negative mutants (P2A or N382S) or an empty vector control (Vector). Error bars indicate SD. *P ≤ .05, **P ≤ .01, ***P ≤ .001.

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