Effect of LBH on mTORC1 and mTORC2. (A-B) DHL-6 and Ly7 DLBCL lines were treated with 100 nM LBH for the indicated times (A) or with the indicated concentrations (B) of LBH for 24 hours and then subjected to Western blot analysis using p-S6 or p-4EBP1 or p-mTOR antibody. (C) Malignant cells from DLBCL patients (n = 4) were treated with 100 nM LBH for 6 hours and analyzed by Western blot. (D) DHL-6 cells were cotreated with 10 nM LBH and 10 nM rapamycin for 24 hours before S6 and 4EBP1 phosphorylation was analyzed. Effect of LBH on mTORC2 targets. (E-F) DHL-6 and Ly7 DLBCL lines were treated with 100 nM LBH for the indicated times (E) or with the indicated concentrations (F) of LBH for 24 hours and then analyzed for Akt or eIF4E phosphorylation. (G) Malignant cells from DLBCL patients (n = 4) were treated with 100 nM LBH for 6 hours and were analyzed for Akt or eIF4E phosphorylation. (H) DHL-6 and Ly7 cells were treated with 100 nM LBH and 20 nM rapamycin for 24 hours before analysis of Akt phosphorylation.