Calpeptin, a calpain inhibitor, partially inhibits AIF release in gossypol-treated CLL primary cells. CLL primary cells were either untreated (U) or treated with 30 μM gossypol (G) in the presence and absence of calpeptin (Cp) for 24 hours, and the expression of AIF was evaluated by immunoblotting in the cytosolic (C), mitochondrial (M), and nuclear (N) fractions. Histone 1 (H1) was used as loading control.