Effect of gossypol on the antiapoptotic proteins of Bcl-2 family members. CLL primary cells were incubated with 10 μM and 30 μM gossypol for the indicated times. Cells were lysed and immunoblotted using antibodies that recognize Mcl-1, Bcl-2, and Bcl-xl. Actin was used as a loading control. Similar experiments were done in 8 patients; data from 3 representative patients are provided (A-C). In panel C the last lane is spliced from the same gel that was originally loaded on lane 8.