Importance of the ADAMTS13 disintegrin-like domain in VWF115 proteolysis. (A) Schematic representation of the domain organization of ADAMTS13, and the truncated variants MP-Dis and MP. (B) ADAMTS13, MP-Dis, and MP detection by Western blotting in conditioned media after transfection using an anti-His mAb. (C) SDS-PAGE and silver staining of MP-Dis and MP after purification. (D) ADAMTS13, MP-Dis, or MP (5 nM each) were incubated with 6 μM VWF115 at 37°C. Reactions were stopped with EDTA and sample was analyzed by SDS-PAGE. (E) Reactions as in panel D except 50 nM MP-Dis or MP was used.