AR-42 modulates Akt and STAT3/5 pathways in malignant mast cells. P815, C2, and BR cells and canine BMCMCs (1μM AR-42 and 17-AAG) were treated with AR-42 (0.5-3μM) or 17-AAG (1μM) for 24 hours. Effects on the expression of phosphorylated and total levels of Akt, STAT3, and STAT5 were determined by Western blotting. Three independent experiments were performed.