Confirmation of novel targets of Scl binding in vivo in primary tissue. (A) Fetal livers were dissected from E11.5 wild-type embryos, and chromatin was prepared and immunoprecipitated using an antibody against Scl. Enriched DNA was then analyzed by quantitative real-time PCR. (B) Real-time PCR analysis of ChIP assays in E11.5 fetal liver (FL) validates Scl binding to previously unknown TF targets of Scl. Fold enrichment levels were normalized to IgG and compared with a negative control region (Runx1 +31kb).