ChIP-Seq analysis recovers known Scl-binding events. (A) Real-time PCR analysis of ChIP assays in HPC-7 cells performed with Scl antibody. Levels of enrichment were normalized to IgG and compared with a negative control region (Runx1 +31kb). (B) Schematic diagram of the intersection and filtering of the ChIP-Seq data (* relevant peak-finding software, either Findpeaks 3.1 or Illumina BeadStudio). (C) Representation of combined ChIP-Seq sequencing reads (raw data) across the Runx1 locus, showing significant binding of Scl at the +23kb region, highlighted by the arrowhead. Sequencing reads are displayed as a custom track on the UCSC genome browser together with the UCSC known genes and vertebrate multiz alignment and conservation tracks.