Detection of new mutations in the second time point sample of MM patients. (A) The positions of the germ-line elements in the V/D/J-S region are indicated at the top, with CS-PCR sequence from the diagnosis time point represented for patients MM1-5 below. Dashed lines represent regions that were not sequenced. New mutations in the second time point sample are shown as follows: closed circles are point mutations with base changes between first and second samples presented directly underneath; open circles, deletions, with the number of bases deleted shown above. A deletion followed by tandem duplication of downstream DNA is shown for MM3. (B) The frequency of each point mutation is summarized in the box.