Associations of peripheral blood CD34⁺ cell count after G-CSF administration and weight according to G-CSF dose per kg donor weight and total amount of G-CSF received. Peripheral blood CD34⁺ cell counts plotted against donor weight (A) were significantly higher in donors who received G-CSF at 16 μg/kg per day given as 8 μg/kg per day twice a day (○, dashed line) than in donors who received 10 μg/kg per day (♦, solid line). In contrast, peripheral blood CD34⁺ cell counts after G-CSF administration plotted against the total dose of G-CSF received (B) were similar in donors who received G-CSF at either 16 μg/kg per day given as 8 μg/kg per day twice a day (○, dashed line) or 10 μg/kg per day (♦, solid line), suggesting that the total amount of G-CSF received exerts a larger impact on CD34⁺ mobilization than the dose of G-CSF administered per kilogram of donor weight.