BALB3T3 and NIH3T3 cells were treated with interferon-α for 18 hours. Expression levels of Ifi202, Ifi203, Ifi204, Ifi205, and Mndal were determined by RT-PCR in the BALB3T3 (A) and NIH3T3 (B) cells. The Mndal gene was found to be absent by sequencing genomic DNA from NIH3T3 cells. (C) Microarray data (mined from the GEO database)²¹  collected from isolated B cells from mice that were either treated with type I interferon or infected with influenza. Mndal expression in B cells isolated from wild-type (+/+) mice that were either infected with influenza or treated with type I interferon-β (Ifn-β) were compared with control. Mndal expression was also examined in B cells isolated from interferon receptor knockout (−/−) mice that had also been infected with influenza.