qPCR quantitation of (whole-lung) pulmonary endothelial antigens. qPCr quantitation in NY1DD mice shows that the stress of H/R, compared with controls at ambient air, does increase level of murine TF mRNA (left panel; P = .023), and does not significantly increase the level of murine VCAM1 mRNA (middle panel; P = .119). In the right panel, level of a control mRNA (murine PECAM) does not change, as expected (P = .383). These measurements are consistent with the protein data shown in Figure 2. To obtain each data point (n = 4 for each antigen), endothelial cells from 3 mice had to be pooled for analysis. Error bars show ±SD.