Ethanol down-regulates mTOR in a TSC2-independent manner. Representative Western blot analysis of (A) Raji and SUDHL-4, (B) B cells and PBLs, and (C) MCF-7, BT-474, and BT-20 cells treated with ethanol (0-20 mM) for 10 days. A total of 50 μg total protein lysates was loaded, and the abundance of pTSC2, TSC2, pAMPK, pAKT, and AKT was assessed.