Genealogic trees. Subclones were identified by sequence alignments and by comparisons between biologic samples. Genealogic trees were then constructed (top panels, where nucleotide changes are given for each clone). Dominant subclones were identified in all samples (bottom panels). For each sample, the biologic type (LN indicates lymph node; PB, peripheral blood; BM, bone marrow; PF, peritoneal fluid; and TR, autologous transplant) and time from diagnosis are indicated. For all patients, the first vertical bar corresponds to diagnosis. When different subclones coexist, minor subclones are presented as small circles. Treatments are indicated by arrows. For patient no. 1131, transplanted cells (TR) were obtained before the first relapse, as indicated by arrows.