Global consequence of gp96 deletion on hematopoiesis. (A) Experimental scheme. (B) Immunoblot of various hematopoietic tissues from WT and KO mice 12 days post-TAM deletion (PTD). Thy indicates thymus. β-Actin serves as loading control. (C) Intracellular staining for gp96 in various hematopoietic tissues from WT (solid line) or KO (dotted line) bone marrow (BM) chimeras using gp96 antibody (open) or isotype control (shaded). (D) Peripheral blood cell counts of WT (n = 3, □) and KO (n = 4, ■) BM chimeras 1 month PTD. (E) Organ cellularity of WT (n = 3, □) and KO (n = 4, ■) BM chimeras 1 month PTD. Ing indicates inguinal; Cer, cervical; and Mes, mesenteric. (F-G) Absolute cell numbers of indicated lineages in BM and spleen of WT (n = 5, □) and KO (n = 6, ■) BM chimeras 3 to 4 weeks PTD (F) and 7 months PTD (G). Data are representative of at least 3 independent experiments. WBC indicates white blood cell; LY, lymphocyte; MO, monocyte; GR, granulocyte; RBC, red blood cell; and PLT, platelet. *P < .05.